Validity of whole genomes sequencing results in neoplasms in precision medicine

Objective: To compare whole genome sequencing (WGS) results in the 100K Genome Project with the results of routine molecular diagnostics in the treatment precision.

Materials and Methods: We analyzed 374 cancer include a high tumor burden mutation (TMB-high) subgroup, defined as> 10 non-synonymous single nucleotide variation per megabase. colon cancer were evaluated for microsatellite instability (MSI), mismatch repair (MMR) genes and NRAS, KRAS and BRAF mutations using molecular diagnostic routine. Fluorescence in situ hybridization / immunohistochemistry were used to evaluate Her2Neu status in breast cancer.

Results: There was a high correlation between the WGS and routine diagnostic test results regardless of the status of TMB in colon cancer. Her2Neu status was discordant in 3 of 5 TMB-high breast cancer (p = 0.049). The presence of ductal carcinoma in-situ correlated significantly with irregularities (p = 0.04). There are 3 (5%) cases of colorectal discordant, all in the KRAS gene, two of which came from non-invasive adenomatous component (p = 0.0058). Of the 374 cases we have identified 24 tumors with TMB> 10; comprising (colorectal carcinoma (CRC) n = 16, n = 5 breast carcinoma, bladder urothelial cell cancer n = 3). Of the 16 high-TMB colorectal adenocarcinoma, 13 have the status of MSI-high. Same 13 has broken MMR protein expression. TMB-high colorectal cancer have 100% results in line between the WGS and NGS testing for KRAS, BRAF and NRAS (16/16).

Conclusion: The microsatellite and colorectal cancer mutation status was evaluated by WGS seems to correlate well with routine diagnostic tests if it’s determined that the invasive component sequencing. WGS evaluation results need to be carefully correlated with histomorphology, such as tumor heterogeneity / contamination with pre-malignant components that need to be taken into account.

Comparative Genome Sequence Analysis of samples of Aspergillus fumigatus Geographic-Relevance to Amphotericin B Resistance

Amphotericin B (AMB) is the main fungicide polyene agent has a broad spectrum of action against invasive fungal infections. AMB is usually used as a last-line drug against serious and life-threatening infections when other drugs have failed to eliminate pathogenic fungi. More recently, resistance in Aspergillus fumigatus AMB has become more evident. For example, high levels of resistance AMB (96%) was recorded in populations of A. fumigatus in Hamilton, Ontario, Canada. AMB resistant strains have also been found in other countries. However, AMB resistance mechanisms are still largely unknown.

Here, we investigated the potential genes and mutations associated with resistance to AMB using whole genome sequences and examined the distribution of AMB resistance between genetic population. A total of 196 sequences representing the entire genome of a strain of the 11 countries examined. Analysis of single nucleotide polymorphisms (SNPs) at the level of the entire genome revealed that this strain belongs to three different genetic groups, with the majority (90%) of AMB resistant strains is situated in one of the three clusters, cluster 2. Our analysis identified more than 60 SNP AMB significantly associated with resistance. Together, these SNPs represent promising candidates from which to investigate the molecular mechanism of resistance is suspected AMB and for their potential use in developing rapid diagnostic marker for clinical screening AMB resistance in A. fumigatus.

 Validity of whole genomes sequencing results in neoplasms in precision medicine
Validity of whole genomes sequencing results in neoplasms in precision medicine

Rate Campylobacter fetus subsp. venerealis molecular diagnosis using clinical samples of bull

Background: Campylobacter fetus subsp. venerealis (CFV) is the pathogen responsible for Bovine Genital Campylobacteriosis (BGC), a venereal disease of cattle that is associated with impaired reproductive performance. Although some developed PCR tests to identify pathogens, most of them still lack evaluated in clinical samples. This study evaluated the real-time PCR tests for detection bull CFV in preputial samples (n = 308).

Results: The detection at the subspecies level (CFV) compared four tests: two targeting ISCfe1 and two PARA gene targeting. Detection at the level of species (C. fetus) is regarded as a test of gene targeting Nahe and commercial kits for the identification of C. fetus. At the subspecies level, tests directed either to a different target (Para and ISCfe1), or for the same target (ISCfe1 or PARA), showed a high percentage of the results agree. All samples were positive at the subspecies level (n = 169) detection tests negative in C. fetus, a strong showing horizontal gene transfer ISCfe1 and PARA to other bacterial species.

NATtrol Human Parvovirus B19 (10,000 IU/mL) (1 mL)

NATPARVO-0001 1 mL
EUR 265.28
Description: Please contact Gentaur in order to receive the datasheet of the product.

NATtrol Human Parvovirus B19 (100,000 IU/mL) (1 mL)

NATPARVO-0002 1 mL
EUR 525.28
Description: Please contact Gentaur in order to receive the datasheet of the product.

NATtrol Human Parvovirus B19 (10,000,000 IU/mL) (1 mL)

NATPARVO-0004 1 mL
EUR 1135.76
Description: Please contact Gentaur in order to receive the datasheet of the product.

NATtrol Human Parvovirus B19 (100,000,000 IU/mL) (1 mL)

NATPARVO-0005 1 mL
EUR 1408.24
Description: Please contact Gentaur in order to receive the datasheet of the product.

Mouse antibody for Parvovirus B19

5191 100 ug
EUR 321.88
Description: This is purified Mouse monoclonal antibody against Parvovirus B19 for WB, ELISA.

Human Rheumatoid Factors IgM (RF) positive control plasma (>400 IU/ml)

3000-PC 1 ml
EUR 103

Parvovirus B19 Antibody

abx023653-1mg 1 mg
EUR 940

Parvovirus B19 VP2 Protein

abx061460-1mg 1 mg
EUR 3265

Parvovirus B19 NS1 Protein

abx061544-1mg 1 mg
EUR 2778

Parvovirus B19 VP1 Protein

abx061545-1mg 1 mg
EUR 2778

Parvovirus B19 Vp1 Protein

abx061562-1mg 1 mg
EUR 3265

Parvovirus B19 VP1 Protein

abx061563-1mg 1 mg
EUR 3265

Parvovirus B19 VP1 Protein

abx061564-1mg 1 mg
EUR 3265

Parvovirus B19 VP2 Protein

abx061566-1mg 1 mg
EUR 3265

Recombinant Parvovirus (MPV) Capsid Protein 2 (VP2) control for Western blot

MPV21-C 100 ul
EUR 286

Anti-Human Parvovirus B19 antibody

STJ16100413 1 mL
EUR 826

Goat IgG (-ve control for flow cytometry) (isotype control)

20011-100 100 test
EUR 103

BSA Control for Age-BSA

2221-BSA
EUR 175

BSA Control for AGE-BSA

35R-AA007 10 mg
EUR 224
Description: BSA Control for AGE protein (BSA modified), Cat No. 30R-AA007

Control/Blocking peptide for Human WNT5a

WNT511-P 100 ug
EUR 164

Mouse Serum albumin control (level 1 ~ 1ug/ml) for ELISA

6300-C1 1 ml
EUR 141

Core Panel Multi-Tumor control slides

TS900 Set of 5
EUR 218

Core Panel Multi-Tumor control slides

TS900-25 Set of 25
EUR 485

Breast Panel Multi-Tumor control slides

TS901 Set of 5
EUR 218

Breast Panel Multi-Tumor control slides

TS901-25 Set of 25
EUR 485

Recombinant (E.coli) Swine/Porcine Parvovirus (PPV) NS1 protein control for western blot

PPVNS11-C 100 ul
EUR 286

Recombinant (E.coli) Swine/Porcine Parvovirus (PPV) VP2 protein control for western blot

PPVVP21-C 100 ul
EUR 286

Human Parvovirus B19 (HPV-B19) IgM Rapid Test Kit

abx092076-20tests 20 tests
EUR 342

Parvovirus B19 VP1/VP2 Protein

abx061565-1mg 1 mg
EUR 2778

Parvovirus B19 VLP VP2 Protein

20-abx262033
  • EUR 4490.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug

Recombinant Parvovirus NS1 Protein (B19)

VAng-Lsx0431-inquire inquire Ask for price
Description: Parvovirus NS1 (B19), recombinant prottein from E. coli.

Recombinant Parvovirus B19 VP2 VLPs

VAng-Lsx0437-inquire inquire Ask for price
Description: Parvovirus B19 VP2 VLPs, recombinant protein from Sf9.

Human parvovirus B19 Capsid protein VP1

1-CSB-BP362102HPM
  • EUR 593.00
  • EUR 255.00
  • EUR 1481.00
  • EUR 791.00
  • EUR 1086.00
  • EUR 347.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human parvovirus B19 Capsid protein VP1,partial expressed in Baculovirus

Human parvovirus B19 Capsid protein VP1

1-CSB-EP362102HPM
  • EUR 380.00
  • EUR 214.00
  • EUR 1309.00
  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human parvovirus B19 Capsid protein VP1,partial expressed in E.coli

Human IgM (Rheumatoid Factor plasma, >40,000 IU/ml, purifed for ELISA)

20007-2-RF 100 ul
EUR 469

Control for HER2, 4 cases (1.5mm)

HRC081 1
EUR 85
Description: IHC control array containing 4 breast cancer cases of strong, moderate, low and negative HER2 breast cancer expressers in duplicates

Control for PR, 3 cases (1.5mm)

PRC061 1
EUR 85
Description: IHC control array containing 3 breast cancer cases of strong, moderate, low/negative progesterone receptor (PR) expressers in duplicates

SLLK, Control Peptide for TSP1 Inhibitor

HY-P0301 1mg
EUR 133

Control for ER, 4 cases (1.5mm)

ERC081 1
EUR 85
Description: IHC control array containing 4 breast cancer cases of strong, moderate, weak/nil estrogen receptor (ER) expressers in duplicates

Control for DOG1, 3 cases (1.5mm)

DOG1061 1
EUR 85
Description: IHC control array containing 3 GIST cases of strong, moderate, weak/nil DOG1 expressers in duplicates

Control for Ckit, 6 samples (1.5mm)

CKIT061 1
EUR 85
Description: Gastrointestinal stroma tumors, 6 cores, 3 cases in duplicates showing strong, moderate and no expression of cKit molecule.

PAS/Glycogen, For Digestion (Control Slides)

TCS0024-100 100 Slides
EUR 706

PAS/Glycogen, For Digestion (Control Slides)

TCS0024-25 25 Slides
EUR 232

PAS/Glycogen, For Digestion (Control Slides)

TCS0024-5 5 Slides
EUR 98

Thiostatin Rat protein, control for western

TSTN11-C 100 ul
EUR 286

Positive Control for Anti-Human CYP1A2 Antibody

P1A2CON 100 uL
EUR 125

Positive Control for Anti-Human CYP2E1 Antibody

P2E1PTCON 100 uL
EUR 125

Positive Control for Anti-Human CYP3A Antibody

P3ACON 100 uL
EUR 125

Positive Control for Anti-Human CYP1B1 Antibody

PH1B1CON 100 uL
EUR 125

Purified, Human S100 protein control for WB

S1002-C 100 ul
EUR 286

Control/Blocking peptide for Human WNT-1

WNT111-P 100 ug
EUR 457

Haptoglobin Human Plasma protein control for WB

HGLB18-C 100 ul
EUR 286

Human Hemopexin purified protein control for Western

HPEX11-C 100 ul
EUR 286

Rec. Human CD21 protein control for WB

CD21-C 100 ul
EUR 286

Human Apolipoprotein B protein control for WB

APOB21-C 100 ul
EUR 286

Ferret IgG (Control, non-immune, isotype control), semi-pure for ELISA

20021-1 100 ug
EUR 225

Elk IgG, semi-pure for ELISA (Control, non-immune, isotype control)

20024-1 100 ug
EUR 225

Deer IgG, semi-pure for ELISA (Control, non-immune, isotype control)

20024-2 100 ug
EUR 225

Bison IgG, semi-pure for ELISA (Control, non-immune, isotype control)

20025-1 100 ug
EUR 225

Raccoon IgG, semi-pure for ELISA (Control, non-immune, isotype control)

20026-1 100 ug
EUR 225

Skunk IgG, semi-pure for ELISA (Control, non-immune, isotype control)

20027-1 100 ug
EUR 225

Holder for Plasmid Midi, Maxi and Maxi plus, Ion Exchange column

FAPDE-holder-for-ion-exchange 1 prep
EUR 158

Swine/Porcine Parvovirus (PPV) IgG negative control serum

AE-200170-01N 1 ml
EUR 164

Swine/Porcine Parvovirus (PPV) IgG positive control serum

AE-200170-02P 1 ml
EUR 225

Recombinant purified Rat Toolan's H1 parvovirus (THV) capsid protein VP2 control for Western blot

THVP11-C 100 ul
EUR 286

human parvovirus B19 IgG,B19IgG ELISA Kit

201-12-2113 96 tests
EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

human parvovirus B19 IgM,B19IgM ELISA Kit

201-12-2114 96 tests
EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human parvovirus B19 IgM(B19IgM)ELISA Kit

QY-E01079 96T
EUR 361

Human parvovirus B19 IgG(B19IgG)ELISA Kit

QY-E01080 96T
EUR 361

Mouse Anti Parvovirus B19 Monoclonal Antibody

DMABT-51485MP 0.1 mg
EUR 892

Recombinant Parvovirus B19 VLP VP2 Protein

VAng-Lsx0432-inquire inquire Ask for price
Description: Parvovirus B19 VP2, recombinant protein from Sf9.

Recombinant Parvovirus B19, VP1, VP2 Protein

VAng-Lsx0433-inquire inquire Ask for price
Description: Recombinant Parvovirus B19 VP1&VP2 protein. Expressed by recombinant baculovirus (Autographa californica multiple nuclear polyhedrosis virus; AcMNPV) infection of Spodoptera frugiperda Sf9 insect cells. Full-length cDNAs coding for human parvovirus B19 VP.

Recombinant Parvovirus B19 NS1 Protein [His]

VAng-Lsx0436-inquire inquire Ask for price
Description: Parvovirus B19 NS1, recombinant protein from E. coli.

Recombinant Parvovirus B19 VP1 Protein [His]

VAng-Lsx0439-inquire inquire Ask for price
Description: Parvovirus B19 VP1, recombinant protein from E. coli.

Positive Control for Anti-Human Cytochrome b5 Antibody

Cb5CON 100 uL
EUR 125

Purified Human Neutrophil Elastase protein control for WB

ESTE11-C 100 ul
EUR 286

Human Salivary Amylase protein positive control for WB

SAMY16-C 100 ul
EUR 286

Purified Human Survivin protein for WB +ve control

SURV11-C 100 ul
EUR 286

Purified, recombinant Human TACE control protein for WB

TACE11-C 100 ul
EUR 286

Human Pancreatic Amylase protein control for Western blot

PAMY13-C 100 ul
EUR 286

Human Prothrombin (Factor II) protein control for Western

PRTN11-C 100 ul
EUR 286

Myoglobin, Human Cardiac, purified protein control for western

MYG17-C 100 ul
EUR 286

Purified human Catalase (CATL) protein control for WB

CATL11-C 100 ul
EUR 286

Human Apolipoprotein C-I protein control for WB

APOC11-C 100 ul
EUR 286

Human Apolipoprotein C-II protein control for WB

APOC22-C 100 ul
EUR 286

Human Apolipoprotein C-III protein control for WB

APOC32-C 100 ul
EUR 286

Human plasma Apolipoprotein E protein control for WB

APOE13-C 100 ul
EUR 286

Human Plasma Apolipoprotein J protein control for WB

APOJ13-C 100 ul
EUR 286

Control Sections for FD NeuroSilver™ Kit

PCS101 each
EUR 58

Positive Control for Anti-Rat MEH Antibody

MEHCON 100 uL
EUR 125

Positive Control for Anti-Rat CYP2B1 Antibody

P2B1PTCON 100 uL
EUR 125

Positive Control for Anti-Rat CYP2C Antibody

P2CCON 100 uL
EUR 125

Positive Control for Anti-Rat CYP2D Antibody

P2DCON 100 uL
EUR 125

Positive Control for Anti-Rat CYP3A2 Antibody

P3A2PTCON 100 uL
EUR 125

SLLK, Control Peptide for TSP1 Inhibitor(TFA)

HY-P0301A 5mg
EUR 380

Mouse IgG (-ve control for flow cytometry)

20008-1-100 100 tests
EUR 103

Rabbit IgG (-ve control for flow cytometry)

20009-1-100 100 tests
EUR 103

Purified Flagellin (salmonella) protein control for WB

FLGN17-C 100 ul
EUR 286

This was confirmed by microbiological isolation of three strains of Campylobacter portucalensis responsible for false positive results. The sequence with a high degree of identity with ISCfe1 and PARA from CFV genes identified in C. portucalensis genome.

Conclusion: Overall, the study revealed that the PCR test is directed solely to the subspecies derived targeting high level of false positive results, due to the presence of homologous sequences ISCfe1 and in other bacterial species, namely from the genus Campylobacter. Although the specificity of these methods may be higher if applied steers from the herd with clinical features BGC or in other geographical areas, PCR diagnosis at this time should be several subspecies and target species, and further research should be considered to identify specific molecular targets CFV.

Related Posts

Leave a Reply

Your email address will not be published.