Validity of whole genomes sequencing results in neoplasms in precision medicine

Objective: To compare whole genome sequencing (WGS) results in the 100K Genome Project with the results of routine molecular diagnostics in the treatment precision.

Materials and Methods: We analyzed 374 cancer include a high tumor burden mutation (TMB-high) subgroup, defined as> 10 non-synonymous single nucleotide variation per megabase. colon cancer were evaluated for microsatellite instability (MSI), mismatch repair (MMR) genes and NRAS, KRAS and BRAF mutations using molecular diagnostic routine. Fluorescence in situ hybridization / immunohistochemistry were used to evaluate Her2Neu status in breast cancer.

Results: There was a high correlation between the WGS and routine diagnostic test results regardless of the status of TMB in colon cancer. Her2Neu status was discordant in 3 of 5 TMB-high breast cancer (p = 0.049). The presence of ductal carcinoma in-situ correlated significantly with irregularities (p = 0.04). There are 3 (5%) cases of colorectal discordant, all in the KRAS gene, two of which came from non-invasive adenomatous component (p = 0.0058). Of the 374 cases we have identified 24 tumors with TMB> 10; comprising (colorectal carcinoma (CRC) n = 16, n = 5 breast carcinoma, bladder urothelial cell cancer n = 3). Of the 16 high-TMB colorectal adenocarcinoma, 13 have the status of MSI-high. Same 13 has broken MMR protein expression. TMB-high colorectal cancer have 100% results in line between the WGS and NGS testing for KRAS, BRAF and NRAS (16/16).

Conclusion: The microsatellite and colorectal cancer mutation status was evaluated by WGS seems to correlate well with routine diagnostic tests if it’s determined that the invasive component sequencing. WGS evaluation results need to be carefully correlated with histomorphology, such as tumor heterogeneity / contamination with pre-malignant components that need to be taken into account.

Comparative Genome Sequence Analysis of samples of Aspergillus fumigatus Geographic-Relevance to Amphotericin B Resistance

Amphotericin B (AMB) is the main fungicide polyene agent has a broad spectrum of action against invasive fungal infections. AMB is usually used as a last-line drug against serious and life-threatening infections when other drugs have failed to eliminate pathogenic fungi. More recently, resistance in Aspergillus fumigatus AMB has become more evident. For example, high levels of resistance AMB (96%) was recorded in populations of A. fumigatus in Hamilton, Ontario, Canada. AMB resistant strains have also been found in other countries. However, AMB resistance mechanisms are still largely unknown.

Here, we investigated the potential genes and mutations associated with resistance to AMB using whole genome sequences and examined the distribution of AMB resistance between genetic population. A total of 196 sequences representing the entire genome of a strain of the 11 countries examined. Analysis of single nucleotide polymorphisms (SNPs) at the level of the entire genome revealed that this strain belongs to three different genetic groups, with the majority (90%) of AMB resistant strains is situated in one of the three clusters, cluster 2. Our analysis identified more than 60 SNP AMB significantly associated with resistance. Together, these SNPs represent promising candidates from which to investigate the molecular mechanism of resistance is suspected AMB and for their potential use in developing rapid diagnostic marker for clinical screening AMB resistance in A. fumigatus.

Validity of whole genomes sequencing results in neoplasms in precision medicine
Validity of whole genomes sequencing results in neoplasms in precision medicine

Rate Campylobacter fetus subsp. venerealis molecular diagnosis using clinical samples of bull

Background: Campylobacter fetus subsp. venerealis (CFV) is the pathogen responsible for Bovine Genital Campylobacteriosis (BGC), a venereal disease of cattle that is associated with impaired reproductive performance. Although some developed PCR tests to identify pathogens, most of them still lack evaluated in clinical samples. This study evaluated the real-time PCR tests for detection bull CFV in preputial samples (n = 308).

Results: The detection at the subspecies level (CFV) compared four tests: two targeting ISCfe1 and two PARA gene targeting. Detection at the level of species (C. fetus) is regarded as a test of gene targeting Nahe and commercial kits for the identification of C. fetus. At the subspecies level, tests directed either to a different target (Para and ISCfe1), or for the same target (ISCfe1 or PARA), showed a high percentage of the results agree. All samples were positive at the subspecies level (n = 169) detection tests negative in C. fetus, a strong showing horizontal gene transfer ISCfe1 and PARA to other bacterial species.

Parvovirus B19 IgM Positive Control
MBS495442-3mLRTU 3mL(RTU)
EUR 160

Parvovirus B19 IgM Positive Control
MBS495442-5x3mLRTU 5x3mL(RTU)
EUR 560

Parvovirus B19 IgG Positive Control
MBS495450-3mLRTU 3mL(RTU)
EUR 160

Parvovirus B19 IgG Positive Control
MBS495450-5x3mLRTU 5x3mL(RTU)
EUR 560

NATtrol Human Parvovirus B19 (1,000,000 IU/mL) (1 mL)
NATPARVO-0003 1 mL
EUR 1177

Recombinant Parvovirus (MPV) Capsid Protein 2 (VP2) control for Western blot
MPV21-C 100 ul
EUR 343.2

Rat Parvovirus Ns1 Positive Control
MBS412140-1mL 1mL
EUR 175

Rat Parvovirus Ns1 Positive Control
MBS412140-5x1mL 5x1mL
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Rat Parvovirus PCR Positive Control
MBS412570-025mL 0.25mL
EUR 1500

Rat Parvovirus PCR Positive Control
MBS412570-5x025mL 5x0.25mL
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NATtrol Human Parvovirus B19 (100,000 IU/mL) (1 mL)
NATPARVO-0002 1 mL
EUR 592

NATtrol Human Parvovirus B19 (10,000,000 IU/mL) (1 mL)
NATPARVO-0004 1 mL
EUR 1353

NATtrol Human Parvovirus B19 (100,000,000 IU/mL) (1 mL)
NATPARVO-0005 1 mL
EUR 1694

Mouse Parvovirus Ns1 Positive Control
MBS412085-1mL 1mL
EUR 175

Mouse Parvovirus Ns1 Positive Control
MBS412085-5x1mL 5x1mL
EUR 645

Mouse Parvovirus IFA Positive Control
MBS412337-1mL 1mL
EUR 150

Mouse Parvovirus IFA Positive Control
MBS412337-5x1mL 5x1mL
EUR 535

Mouse parvovirus PCR Positive Control
MBS412574-025mL 0.25mL
EUR 1500

Mouse parvovirus PCR Positive Control
MBS412574-5x025mL 5x0.25mL
EUR 6545

NATtrol Human Parvovirus B19 (10,000 IU/mL) (1 mL)
NATPARVO-0001 1 mL
EUR 261

Toolan's H-1 PCR Control, Parvovirus
MBS412558-025mL 0.25mL
EUR 1500

Toolan's H-1 PCR Control, Parvovirus
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Mouse Parvovirus Native Antigen Positive Control
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Mouse Parvovirus Native Antigen Positive Control
MBS412084-5x1mL 5x1mL
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Recombinant (E.coli) Swine/Porcine Parvovirus (PPV) NS1 protein control for western blot
PPVNS11-C 100 ul
EUR 343.2

Recombinant (E.coli) Swine/Porcine Parvovirus (PPV) VP2 protein control for western blot
PPVVP21-C 100 ul
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Kilham Rat Virus PCR Control, Parvovirus
MBS412557-025mL 0.25mL
EUR 1500

Kilham Rat Virus PCR Control, Parvovirus
MBS412557-5x025mL 5x0.25mL
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Rat Parvovirus (RPV), Native Antigen Positive Control
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EUR 175

Rat Parvovirus (RPV), Native Antigen Positive Control
MBS412082-5x1mL 5x1mL
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Mouse Parvovirus (MPV1 & MPV2) VP2 Positive Control
MBS412162-1mL 1mL
EUR 175

Mouse Parvovirus (MPV1 & MPV2) VP2 Positive Control
MBS412162-5x1mL 5x1mL
EUR 645

Minute Virus of Mice (MVM) PCR Control, Parvovirus
MBS412553-025mL 0.25mL
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Minute Virus of Mice (MVM) PCR Control, Parvovirus
MBS412553-5x025mL 5x0.25mL
EUR 6545

Recombinant purified Rat Toolan's H1 parvovirus (THV) capsid protein VP2 control for Western blot
THVP11-C 100 ul
EUR 343.2

genesig Easy kit for Human Parvovirus B19
Z-Path-HPVB19-EASY 50 tests
EUR 410
Description: HPVB19

Swine/Porcine Parvovirus (PPV) IgG negative control serum
AE-200170-01N 1 ml
EUR 196.8

Swine/Porcine Parvovirus (PPV) IgG positive control serum
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Parvovirus B19 Real-TM Real Time PCR test for detection of Parvovirus B19
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EUR 516.66

Parvovirus B19, Human
MBS601255-01mg 0.1mg
EUR 575

Parvovirus B19, Human
MBS601255-5x01mg 5x0.1mg
EUR 2440

Mouse Anti- Parvovirus Capsid Protein 1 (MPV-VP1) antibody negative control serum
MPV11-MNC 1 ml
EUR 270

Mouse Anti- Parvovirus Capsid Protein 1 (MPV-VP1) antibody positive control serum
MPV11-MPC 1 ml
EUR 270

Rat Anti-Toolan's H1 parvovirus (THV) capsid protein VP2 antibody negative control serum
THVP11-RNC 1 ml
EUR 196.8

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THVP11-RPC 1 ml
EUR 270

Parvovirus B19, Human (AP)
MBS6241776-01mL 0.1(mL
EUR 860

Parvovirus B19, Human (AP)
MBS6241776-5x01mL 5x0.1mL
EUR 3725

Parvovirus B19, Human (PE)
MBS6244440-01mL 0.1(mL
EUR 860

Parvovirus B19, Human (PE)
MBS6244440-5x01mL 5x0.1mL
EUR 3725

Parvovirus B19, Human (APC)
MBS6242309-01mL 0.1(mL
EUR 860

Parvovirus B19, Human (APC)
MBS6242309-5x01mL 5x0.1mL
EUR 3725

Parvovirus B19, Human (HRP)
MBS6243907-01mL 0.1(mL
EUR 860

Parvovirus B19, Human (HRP)
MBS6243907-5x01mL 5x0.1mL
EUR 3725

Parvovirus B19, Human (FITC)
MBS6243374-01mL 0.1(mL
EUR 860

Parvovirus B19, Human (FITC)
MBS6243374-5x01mL 5x0.1mL
EUR 3725

Parvovirus B19, Human (Biotin)
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Parvovirus B19, Human (Biotin)
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Human Parvovirus B19 ELISA Kit
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Human Parvovirus B19 ELISA Kit
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Parvovirus B19, Human, VP1/VP2
MBS600339-01mg 0.1mg
EUR 835

Parvovirus B19, Human, VP1/VP2
MBS600339-5x01mg 5x0.1mg
EUR 3610

Human Parvovirus B19, B19 ELISA Kit (Qualitative)
SL3635Hu 96 Tests
EUR 468

Human parvovirus B19 IgG,B19 IgG ELISA KIT
ED0458Hu 96 wells
EUR 458

Human parvovirus B19 IgM,B19 IgM ELISA KIT
ED0459Hu 96 wells
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Human parvovirus B19 IgG,B19 IgG ELISA KIT
JOT-EKD0458Hu 96 wells
EUR 366.4
Description: Human

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JOT-EKD0459Hu 96 wells
EUR 366.4
Description: Human

Human parvovirus B19 IgG, B19 IgG ELISA Kit
MBS1610751-10x96StripWells 10x96-Strip-Wells
EUR 3460

Human parvovirus B19 IgG, B19 IgG ELISA Kit
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Human parvovirus B19 IgG, B19 IgG ELISA Kit
MBS1610751-96StripWells 96-Strip-Wells
EUR 425

Human parvovirus B19 IgM(B19-IgM)ELISA Kit
SL2655Hu 96 Tests
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Parvovirus B19, Human (MaxLight 550)
MBS6246039-01mL 0.1(mL
EUR 860

Parvovirus B19, Human (MaxLight 550)
MBS6246039-5x01mL 5x0.1mL
EUR 3725

Parvovirus B19, Human (MaxLight 650)
MBS6246572-01mL 0.1(mL
EUR 860

Parvovirus B19, Human (MaxLight 650)
MBS6246572-5x01mL 5x0.1mL
EUR 3725

Parvovirus B19, Human (MaxLight 750)
MBS6247105-01mL 0.1(mL
EUR 860

Parvovirus B19, Human (MaxLight 750)
MBS6247105-5x01mL 5x0.1mL
EUR 3725

Parvovirus B19, Human (MaxLight 405)
MBS6244973-01mL 0.1(mL
EUR 860

Parvovirus B19, Human (MaxLight 405)
MBS6244973-5x01mL 5x0.1mL
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Parvovirus B19, Human (MaxLight 490)
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Human Parvovirus B19 (HPV-B19) IgG ELISA Kit
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Human Parvovirus B19 (HPV-B19) IgM ELISA Kit
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Human Parvovirus B19 (HPV-B19) IgM ELISA Kit
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MBS2568076-96StripWells 96-Strip-Wells
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Parvovirus B19 IgG
DE3503 96
EUR 179

Parvovirus B19 IgM
DE3504 96
EUR 179

Parvovirus B19 VP2
MBS319611-1mg 1mg
EUR 955

Parvovirus B19 VP2
MBS319611-5x1mg 5x1mg
EUR 4115

Parvovirus B19 VP1
PR-BA122VP1-L 1mg
EUR 1466.72
Description: recombinant, E. coli

Parvovirus B19 VP1
PR-BA122VP1-S 250µg
EUR 488.83
Description: recombinant, E. coli

Human Parvovirus B19 IgM ELISA Kit
MBS495436-5x96Tests 5x96Tests
EUR 2125

Human Parvovirus B19 IgM ELISA Kit
MBS495436-96Tests 96Tests
EUR 465

human anti-Human Parvovirus B19 IgG
E4A11A53-G 50ug
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

human anti-Human Parvovirus B19 IgM
E4A11A53-M 50ug
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

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abx023653-1mg 1 mg
EUR 1128

Parvovirus B19 Antibody
abx023653-400l 400 µl Ask for price

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EUR 1200

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EUR 410.4

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ED0604Hu 96 wells
EUR 458

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JOT-EKD0604Hu 96 wells
EUR 366.4
Description: Human

Human parvovirus B19 Capsid protein VP1
1-CSB-BP362102HPM
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  • 1MG
Description: Recombinant Human parvovirus B19 Capsid protein VP1,partial expressed in Baculovirus

Human parvovirus B19 Capsid protein VP1
1-CSB-EP362102HPM
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  • 1MG
Description: Recombinant Human parvovirus B19 Capsid protein VP1,partial expressed in E.coli

Human parvovirus B19 IgG(B19-IgG)ELISA Kit (Qualitative)
SL2654Hu 96 Tests
EUR 468

Human Parvovirus B19 IgG (B19-IgG) ELISA Kit (Quantitative)
SL2654Hu_1 96 Tests Ask for price

Human Parvovirus B19 IgM (B19-IgM) ELISA Kit (Quantitative)
SL2655Hu_1 96 Tests Ask for price

Parvovirus B19 VLP (VP2)
CBS-V087 Each
EUR 851.9
Description: VLP

Parvovirus B19 VLP (VP1)
CBS-V930 Each
EUR 914.02
Description: VLP

Parvovirus B19 VLP VP2
PR-1277 20µg
EUR 259.72
Description: recombinant, Sf9 insect cells

Parvovirus B19 VLP VP2
prv-001 5µg
EUR 60
Description: Recombinant Parvovirus B19 VLP VP2

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prv-003 100µg
EUR 165
Description: Recombinant Parvovirus VLP VP1

human parvovirus B19 IgG,B19IgG ELISA Kit
201-12-2113 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

human parvovirus B19 IgM,B19IgM ELISA Kit
201-12-2114 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Parvovirus B19 IgG, B19IgG ELISA Kit
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Human Parvovirus B19 IgG, B19IgG ELISA Kit
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Human parvovirus B19 IgG, B19IgG ELISA Kit
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Human parvovirus B19 IgG, B19IgG ELISA Kit
MBS9304327-96StripWells 96-Strip-Wells
EUR 765

Parvovirus B19 VP2 VLPs
PR-BA122VP2-L 1mg
EUR 1731.09
Description: recombinant, Sf9 insect cells

Parvovirus B19 VP2 VLPs
PR-BA122VP2-S 250µg
EUR 576.68
Description: recombinant, Sf9 insect cells

Parvovirus B19 IgG ELISA
MBS494069-5x96Tests 5x96Tests
EUR 3050

Parvovirus B19 IgG ELISA
MBS494069-96Tests 96Tests
EUR 665

Parvovirus B19 IgM ELISA
MBS494186-5x96Tests 5x96Tests
EUR 3050

Parvovirus B19 IgM ELISA
MBS494186-96Tests 96Tests
EUR 665

Parvovirus B19 VLP VP1VP2
prv-002 5µg
EUR 60
Description: Recombinant Parvovirus VLP VP1VP2 Co-Capsid

Parvovirus B19 NS1 Protein
abx061544-100g 100 µg Ask for price

Parvovirus B19 NS1 Protein
abx061544-1mg 1 mg
EUR 3333.6

Parvovirus B19 NS1 Protein
abx061544-200g 200 µg Ask for price

Parvovirus B19 NS1 Protein
abx061544-50g 50 µg
EUR 3512.5

Parvovirus B19 VP1 Protein
abx061545-100g 100 µg Ask for price

Parvovirus B19 VP1 Protein
abx061545-10g 10 µg
EUR 3512.5

Parvovirus B19 VP1 Protein
abx061545-1mg 1 mg
EUR 3333.6

Parvovirus B19 VP1 Protein
abx061545-50g 50 µg Ask for price

Parvovirus B19 Vp1 Protein
abx061562-100g 100 µg Ask for price

Parvovirus B19 Vp1 Protein
abx061562-10g 10 µg
EUR 4187.5

Parvovirus B19 Vp1 Protein
abx061562-1mg 1 mg
EUR 3918

Parvovirus B19 Vp1 Protein
abx061562-50g 50 µg Ask for price

Parvovirus B19 VP1 Protein
abx061563-100g 100 µg Ask for price

Parvovirus B19 VP1 Protein
abx061563-10g 10 µg
EUR 4187.5

Parvovirus B19 VP1 Protein
abx061563-1mg 1 mg
EUR 3918

Parvovirus B19 VP1 Protein
abx061563-50g 50 µg Ask for price

Parvovirus B19 VP1 Protein
abx061564-100g 100 µg Ask for price

Parvovirus B19 VP1 Protein
abx061564-10g 10 µg
EUR 4187.5

Parvovirus B19 VP1 Protein
abx061564-1mg 1 mg
EUR 3918

Parvovirus B19 VP1 Protein
abx061564-50g 50 µg Ask for price

Parvovirus B19 VP2 Protein
abx061566-100g 100 µg Ask for price

Parvovirus B19 VP2 Protein
abx061566-10g 10 µg
EUR 4312.5

Parvovirus B19 VP2 Protein
abx061566-1mg 1 mg
EUR 3918

Parvovirus B19 VP2 Protein
abx061566-50g 50 µg Ask for price

Parvovirus B19 VP2 Protein
abx061460-100g 100 µg Ask for price

Parvovirus B19 VP2 Protein
abx061460-1mg 1 mg
EUR 3918

Parvovirus B19 VP2 Protein
abx061460-200g 200 µg Ask for price

Parvovirus B19 VP2 Protein
abx061460-50g 50 µg
EUR 3962.5

Parvovirus B19 NS1 Antigen
BA122VSNS1 1mg
EUR 1138

Parvovirus B19 VP1 Antigen
BA122VSVP1 1mg
EUR 1138

Parvovirus B19 VP2 Antigen
BA122VSVP1E 1mg
EUR 1348

Parvovirus B19 VLP Antigen
BA122VSVP2 1mg
EUR 1348

Myc-DDK-tagged ORF clone of viral ORF for protein X [Human parvovirus B19], codon optimized for human cell expression, YP_004928147
VC102085 10 µg Ask for price

Human Parvovirus B19-IgG Antibody ELISA Kit
MBS3802580-10x96StripWells 10x96-Strip-Wells
EUR 6725

Human Parvovirus B19-IgG Antibody ELISA Kit
MBS3802580-48StripWells 48-Strip-Wells
EUR 550

Human Parvovirus B19-IgG Antibody ELISA Kit
MBS3802580-5x96StripWells 5x96-Strip-Wells
EUR 3420

Human Parvovirus B19-IgG Antibody ELISA Kit
MBS3802580-96StripWells 96-Strip-Wells
EUR 765

Human parvovirus B19 IgM-B19IgM-ELISA Kit
QY-E01079 96T
EUR 400

Human parvovirus B19 IgG-B19IgG-ELISA Kit
QY-E01080 96T
EUR 400

Anti-Human Parvovirus B19 antibody
STJ16100413 1 mL
EUR 991.2

This was confirmed by microbiological isolation of three strains of Campylobacter portucalensis responsible for false positive results. The sequence with a high degree of identity with ISCfe1 and PARA from CFV genes identified in C. portucalensis genome.

Conclusion: Overall, the study revealed that the PCR test is directed solely to the subspecies derived targeting high level of false positive results, due to the presence of homologous sequences ISCfe1 and in other bacterial species, namely from the genus Campylobacter. Although the specificity of these methods may be higher if applied steers from the herd with clinical features BGC or in other geographical areas, PCR diagnosis at this time should be several subspecies and target species, and further research should be considered to identify specific molecular targets CFV.

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NCBI References of Lab PCR Assays

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