Mucormycosis is a fungal infection angioinvasive, because fungi of the Mucorales order. incidents that can not be measured precisely, because there are few population-based studies, but some studies have shown that it increases. Mucormycosis prevalence in India is approximately 80 times the prevalence in developed countries, to about 0.14 cases per 1000 population. Diabetes mellitus is a major underlying disease globally, especially in low- and middle-countries. In developed countries the underlying disease is the most common hematologic malignancy and transplantation.
Τhe mucormycosis epidemiology evolved as a new immunomodulatory agent used in the treatment of cancer and autoimmune diseases, and as a modern diagnostic tools lead to earlier identification of rare genera / species like Apophysomyces or Saksenaea complex. Additionally, new risk factors reported from Asia, including post-pulmonary tuberculosis and chronic kidney disease. New species arise including Rhizopus homothallicus, Thamnostylum lucknowense, irregularis Mucor and Saksenaea erythrospora. Diagnosis remains of mucormycosis challenging. Clinical approach to diagnosis has a sensitivity and low specificity, but helps in increasing suspicion and encourage initiation of laboratory testing.
Histopathology, direct examination and culture remain important tools, although improved molecular methods. Internal transcribed spacer region (ITS) is the most widely sequencing of DNA regions of the fungus and is recommended as first-line method for the identification of the species Mucorales. new molecular platform that is being investigated and new genetic targets mushrooms are being explored. molecular-based methods have gained acceptance to confirm infection when applied to the network. Mucorales detection method on the DNA in the blood have shown promising results for the early diagnosis and quick and can be used as a screening test in high-risk patients, but it has been validated in clinical studies. More, much needed, fast method that does not require invasive procedures, such as breath test metabolomics-based point-of-care, or based on serology, are being developed and hopefully will be evaluated in the near future.
Epidemiology and Diagnosis of Mucormycosis: An Update
Clinical analysis of network-connected nature expresses markers of psoriasis treatment of active transcription with Liangxue-Jiedu decoction
Ethnopharmacological relevance: Psoriasis is a recurrent inflammatory skin disease complex with different pathological changes in various stages. Psoriasis in the active phase, which is comparable to the kind of blood-heat in traditional Chinese medicine (TCM), has been conventionally treated by Liangxue Jiedu decoction (LJD) in TCM for decades with proven success. According to TCM theory, LJD has the function of removing heat and pathogenic factors from the blood.
The purpose of this research: We aimed to determine the molecular features associated with blood-heat syndrome active psoriasis and to identify genes respond to treatment LJD accompanied by a lesion remission.
Materials and methods: healthy volunteers and patients with psoriasis who meet the diagnostic criteria specifically recruited. Twenty-six profiled transcriptome of cells of peripheral blood mononuclear (PBMC) from 10 patients with psoriasis (pre- and post-treatment) and 6 healthy volunteers.
Data sequencing RNA is analyzed using an integrated approach which combines differential analysis of gene expression (DGEA) and network analysis of gene co-expression of weighted (WGCNA), where the expression of genes associated with some clinical characteristics, including the psoriasis and severity index (PASI), and the increased rate of skin lesions (ΔPASI). LJD action then verified using in vitro cell assay combined flow cytometric analysis and RT-PCR.
NATtrol GBS Negative Control (6 X 0.5 mL)
NATLAC-6MC-IVD
Zeptometrix
6 X 0.5 mL
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NATtrol MRSA Positive Control (6 X 0.5 mL)
NATMRSA-6MC-IVD
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20011-100
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Alpha Diagnostics
100 ul
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100 ul
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100 ug
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100 ul
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1
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MedChemExpress
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Pantomics
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ScyTek Laboratories
100 Slides
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ScyTek Laboratories
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Alpha Diagnostics
100 ul
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NSJ Bioreagents
100ug
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V8785-20UG
NSJ Bioreagents
20ug
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Negative Control Antibody for Mouse Monoclonals
V8785SAF-100UG
NSJ Bioreagents
100ug
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Deer IgG, semi-pure for ELISA (Control, non-immune, isotype control)
20024-2
Alpha Diagnostics
100 ug
EUR 270
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Cell Biolabs
50 ?L
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Cell Biolabs
50 ?L
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AAV-303
Cell Biolabs
50 ?L
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AAV-304
Cell Biolabs
50 ?L
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AAV-305
Cell Biolabs
50 ?L
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AAV-306
Cell Biolabs
50 ?L
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AAV-310
Cell Biolabs
50 ?L
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AAV-311
Cell Biolabs
50 ?L
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AAV-313
Cell Biolabs
50 ?L
EUR 1221.6
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AAV-314
Cell Biolabs
50 ?L
EUR 1221.6
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AAV-315
Cell Biolabs
50 ?L
EUR 1221.6
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AAV-316
Cell Biolabs
50 ?L
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AAV-320
Cell Biolabs
50 ?L
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AAV-321
Cell Biolabs
50 ?L
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AAV-323
Cell Biolabs
50 ?L
EUR 1221.6
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AAV-324
Cell Biolabs
50 ?L
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AAV-325
Cell Biolabs
50 ?L
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AAV-326
Cell Biolabs
50 ?L
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AAV-331
Cell Biolabs
50 ?L
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AAV-332
Cell Biolabs
50 ?L
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AAV-333
Cell Biolabs
50 ?L
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AAV-334
Cell Biolabs
50 ?L
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AAV-335
Cell Biolabs
50 ?L
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AAV-336
Cell Biolabs
50 ?L
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AAV-341
Cell Biolabs
50 ?L
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AAV-342
Cell Biolabs
50 ?L
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AAV-343
Cell Biolabs
50 ?L
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Cell Biolabs
50 ?L
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AAV-345
Cell Biolabs
50 ?L
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Cell Biolabs
50 ?L
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AAV-351
Cell Biolabs
50 ?L
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AAV-352
Cell Biolabs
50 ?L
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AAV-353
Cell Biolabs
50 ?L
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AAV-354
Cell Biolabs
50 ?L
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AAV-355
Cell Biolabs
50 ?L
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AAV6 Null Control Virus
AAV-356
Cell Biolabs
50 ?L
EUR 1221.6
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Holder for Plasmid Midi, Maxi and Maxi plus, Ion Exchange column
FAPDE-holder-for-ion-exchange
Favorgen
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EUR 189.6
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Alpha Diagnostics
100 ul
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Alpha Diagnostics
100 ul
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Alpha Diagnostics
100 ul
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20008-1-100
Alpha Diagnostics
100 tests
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20009-1-100
Alpha Diagnostics
100 tests
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Alpha Diagnostics
100 ul
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Mouse Ceruloplasmin protein control for western blot
CP16-C
Alpha Diagnostics
100 ul
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Alpha Diagnostics
100 ul
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Alpha Diagnostics
100 ul
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Alpha Diagnostics
100 ul
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Mouse negative control sera (purified) for IHC
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Innovex
7 ml
EUR 238.8
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NIVE2B-C
Alpha Diagnostics
100 ug
EUR 196.8
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Detroit R&D
100 uL
EUR 150
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Alpha Diagnostics
100 ul
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Alpha Diagnostics
100 ul
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Alpha Diagnostics
100 ul
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Alpha Diagnostics
100 ul
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Alpha Diagnostics
100 ul
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SLLK, Control Peptide for TSP1 Inhibitor(TFA)
HY-P0301A
MedChemExpress
5mg
EUR 456
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Detroit R&D
100 uL
EUR 150
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Detroit R&D
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100 uL
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Detroit R&D
100 uL
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100 uL
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Detroit R&D
100 uL
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Detroit R&D
100 uL
EUR 150
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Detroit R&D
100 uL
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Alpha Diagnostics
100 ul
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Alpha Diagnostics
100 ul
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Alpha Diagnostics
100 ug
EUR 196.8
Control siRNA Vector (pGB-control)
9500C-20
Biovision
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Alpha Diagnostics
100 ul
EUR 343.2
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Alpha Diagnostics
100 ul
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Recombinant Zika Virus Envelop domain III (African) control for Western blot
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Alpha Diagnostics
100 ul
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Alpha Diagnostics
100 ul
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pRedZeo-CMV Virus [positive control]
SR10046VA-1
SBI
>2 x 10^6 IFUs
EUR 826.8
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SR10052VA-1
SBI
>2 x 10^6 IFUs
EUR 826.8
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SR500VA-1
SBI
>2 x 10^6 IFUs
EUR 806.4
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SR501VA-1
SBI
>2 x 10^6 IFUs
EUR 806.4
Human Apolipoprotein C-I protein control for WB
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Alpha Diagnostics
100 ul
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Alpha Diagnostics
100 ul
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Alpha Diagnostics
100 ul
EUR 343.2
Human plasma Apolipoprotein E protein control for WB
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Alpha Diagnostics
100 ul
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Human Plasma Apolipoprotein J protein control for WB
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Alpha Diagnostics
100 ul
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Chicken Serum Albumin protein control for Western blot
ALBC15-C
Alpha Diagnostics
100 ul
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Monkey (Rhesus) Serum Albumin protein control for WB
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Alpha Diagnostics
100 ul
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Monkey (Rhesus) Serum Albumin protein control for WB
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Alpha Diagnostics
100 ul
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Recombinant Beta Lactamase protein control for Western blot
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Alpha Diagnostics
100 ul
EUR 343.2
Purified human Catalase (CATL) protein control for WB
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Alpha Diagnostics
100 ul
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Positive Control for Anti-Human Cytochrome b5 Antibody
Cb5CON
Detroit R&D
100 uL
EUR 150
Chicken Egg yellow proteins total control for Western
CEYP12-C
Alpha Diagnostics
100 ul
EUR 343.2
Purified Cholera Toxin protein control for Western Blot
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Alpha Diagnostics
100 ul
EUR 343.2
Chicken Egg Conalbumin (ovotransferrin) protein control for Western
CECA15-C
Alpha Diagnostics
100 ul
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Chicken Egg white proteins total control for Western
CEGW12-C
Alpha Diagnostics
100 ul
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Chicken Ovomucoid (trypsin inhibitor) protein control for Western
COVM13-C
Alpha Diagnostics
100 ul
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Rabbit Beta Actin (muscle) protein control for Western
ACTB15-C
Alpha Diagnostics
100 ul
EUR 388.8
Myoglobin, Human Cardiac, purified protein control for western
MYG17-C
Alpha Diagnostics
100 ul
EUR 343.2
Purified Lassa fever Nucleoprotein control for Western Blotting
LFNP15-C-10
Alpha Diagnostics
100 ul
EUR 343.2
Control/Blocking peptide for Mouse Histone deacetylase 1
HDAC111-P
Alpha Diagnostics
100 ug
EUR 196.8
Purified Human Neutrophil Elastase protein control for WB
ESTE11-C
Alpha Diagnostics
100 ul
EUR 343.2
Positive Control for Anti-Rat CYP1A1/1A2 Antibody
P1ACON
Detroit R&D
100 uL
EUR 150
Positive Control for Anti-Rat CHP2B1/2 Antibody
P2B12PTCON
Detroit R&D
100 uL
EUR 150
Human Prothrombin (Factor II) protein control for Western
PRTN11-C
Alpha Diagnostics
100 ul
EUR 343.2
Mouse Prothrombin (Factor II) protein control for Western
PRTN13-C
Alpha Diagnostics
100 ul
EUR 343.2
Control sections for FD NeuroApop™ Kit (paraffin)
PCS102-1
FD Neurotechnologies
each
EUR 80.44
Description: Best quality products
Control sections for FD NeuroApop™ Kit (frozen)
PCS102-2
FD Neurotechnologies
each
EUR 80.54
Description: Best quality products
Human Pancreatic Amylase protein control for Western blot
PAMY13-C
Alpha Diagnostics
100 ul
EUR 343.2
Human Salivary Amylase protein positive control for WB
SAMY16-C
Alpha Diagnostics
100 ul
EUR 343.2
Purified, recombinant Human TACE control protein for WB
TACE11-C
Alpha Diagnostics
100 ul
EUR 343.2
Purified Human Survivin protein for WB +ve control
SURV11-C
Alpha Diagnostics
100 ul
EUR 343.2
Quality Control
abx098966-1vial
Abbexa
1 vial
EUR 360
Recombinant African Swine fever virus (ASFV) P30 protein control for western blot
ASFV11-C
Alpha Diagnostics
100 ul
EUR 343.2
Bovine Viral Diarrhea Virus 1 (BVDV1) Erns Protein control for western blot
BVDR12-C
Alpha Diagnostics
100 ul
EUR 343.2
Recombinant Zaire-Ebola virus nucleoprotein (Mayinga EBOV NP) protein control for Western
EVNP11-C
Alpha Diagnostics
100 ul
EUR 343.2
Recombinant (E.Coli) Japanese Encephalitis Virus (JEV) prM protein control for Western blot
JEV13-C
Alpha Diagnostics
100 ul
EUR 343.2
Recombinant (HEK) Rift Valley Fever Virus NP protein control for Western blot
RVFNP12-C
Alpha Diagnostics
100 ul
EUR 343.2
Recombinant (sf9) Purified Sudan-Ebola virus glycoprotein protein control for Western Blot
SVGP21-C
Alpha Diagnostics
100 ul
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Recombinant (E. coli) Zika Virus Envelop Protein (African) control for Western blot
ZENV11-C
Alpha Diagnostics
100 ul
EUR 343.2
Recombinant (E. coli) Zika Virus NS1 Protein (African) control for Western blot
ZNS111-C
Alpha Diagnostics
100 ul
EUR 343.2
Recombinant Polyoma Virus (KV, Pneumotropic virus) Capsid Protein 1 (VP1) control for Western blot
KVP14-C
Alpha Diagnostics
100 ul
EUR 343.2
NATtrol BK Virus External Run Control, Low (6 X 1 mL)
NATBK-ERCL
Zeptometrix
6 X 1 mL
EUR 406.94
Description: Please contact Gentaur in order to receive the datasheet of the product.
NATtrol BK Virus External Run Control, Medium (6 X 1 mL)
NATBK-ERCM
Zeptometrix
6 X 1 mL
EUR 481.82
Description: Please contact Gentaur in order to receive the datasheet of the product.
Rat neurofascin (Nfasc)-control Control/blocking peptide
AB-23249-CP
Alpha Diagnostics
100ug
EUR 196.8
Alpha 2-Macroglobulin (A2M), Human protein control for WB
A2MG11-C
Alpha Diagnostics
100 ul
EUR 343.2
Alpha-1-Acid Glycoprotein Protein, Dog, control for western
A1AG17-C
Alpha Diagnostics
100 ul
EUR 343.2
Alpha-1-Acid Glycoprotein Protein, Mouse, control for western
A1AG18-C
Alpha Diagnostics
100 ul
EUR 343.2
Results: We identified four network modules with statistical significance (P <0.05), two of which are connected to the PASI score, while the other two are connected to the 8-week treatment and ΔPASI. In patients with psoriasis, inflammatory pathway and inhibit activated G-protein signaling genes (GTPase IMAP family members and the G protein-coupled receptors) co-occur, including high expression of CD83 and CD69 and low expression of CD160 and CD180, compared to healthy controls.
Accompanying LJD treatment and remission of lesions, the expression of CD69 and associated cell cycle genes including CCNA2, CCNB2, Cdk1, and TOP2A down-regulated. LJD inhibitory role on CD69 expression was confirmed by activating naive CD4 + T lymphocytes
Conclusions: Our study suggests that psoriasis is marked with immune status is not balanced with dendritic cell activation and inflammation associated lymphocytes as well as NK-cells and B cell-related defense response aberrance. LJD play a role in the inhibition of T cell activation, a process which is located downstream of the pathological cascade of psoriasis.