Laboratory diagnosis of COVID-19 in China: A review of challenging cases and analysis

Since the early emergence of coronavirus disease in 2019 (COVID-19) in Wuhan, Hubei province, China, the rapid spread of the disease occurs throughout the world, rose to international global health concern at the level of a pandemic. In the face of these medical challenges threaten humans, the development of fast and accurate method for early screening and diagnosis COVID-19 be important to contain the emerging public health threats, and prevent further spread in the population.

Despite the large number COVID 19 confirmed cases in China, some problematic cases with laboratory test results are not consistent, it was reported. In particular, the rate of false-negative high of 41% in the second acute respiratory syndrome coronavirus (SARS-CoV-2) detection by a chain reaction of reverse transcription-polymerase (qRT-PCR) assay real-time observed in China. Although serological testing has been implemented worldwide as a complementary method to help identify the SARS-CoV-2, some limitations on its use have been reported in China. Therefore, the use of the qRT-PCR and blood tests in the diagnosis COVID-19 in China and elsewhere, presented a considerable challenge, but when used in combination, can be a valuable tool in the fight against COVID-19.

In this review, we provide an overview of the advantages and disadvantages of molecular techniques different for SARS-CoV-2 detection currently used in several laboratories, including qRT-PCR, gene sequencing, loop-mediated isothermal amplification (LAMP), nucleic spectrometry acid mass (MS), and gene editing technique based clustered regularly interspaced short palindromic repeats (CRISPR / Cas13) systems. Then we mainly review and analyze some of the causes of false negative results of qRT-PCR, and how to resolve some of the diagnostic dilemma.

Advances in Personalized Medicine and Non-Invasive Diagnostics in Solid Organ Transplantation

personalized medicine has become a mainstay and in practice in transplantation pharmacotherapy since the emergence of the field. Decisions relating to the diagnosis, selection, and monitoring of pharmacotherapy directed against individual transplantation, allograft and immunological needs of all patients. recent advances in pharmacogenomics, non-invasive biomarkers and artificial intelligence technology has the promise to change the way we individualize treatment and monitoring allograft function.

Pharmacogenomic testing can provide physicians with additional data to minimize toxicity and maximize the therapeutic dose in patients at high risk, causing more informed decisions that can lower the risk of rejection and adverse outcomes associated with immunosuppressive therapy. Development of non-invasive strategy for monitoring allograft function may offer a safer method and more convenient for detecting allograft injury. Cell-free DNA and gene expression profiles offer the potential to serve as a “liquid biopsy” minimize the risks for patients and provide clinicians with a useful molecular data that can help individualize immunosuppression and treatment of rejection.

The use of big data in transplantation and new artificial intelligence platform, such as iBox, hold tremendous promise in giving the doctor a “glimpse into the future” so as to allow for a more individualized approach to immunosuppressive therapy which may minimize future adverse outcome. Advances in diagnostic, laboratory science, and artificial intelligence have been made more even application of personalized medicine tailored for solid organ transplant recipients. In this perspective, we summarize current and emerging tools are available, supporting the use of literature, and to personalize the future horizon transplant.

Prospective Evaluation of Single Nucleotide Variants by Two Different Technologies in Paraffin Sample Non-Small Cell Advanced Lung Cancer Patients

targeted therapy is a new paradigm in the management of lung cancer. Next-generation sequencing (NGS) technique has allowed for the simultaneous testing of multiple genes in a way that is fast and efficient; However, there are other molecular diagnostic tools such as 3D Vantage nCounter® single nucleotide variants (SNVs) solid tumor panel that also offers important benefits of the input sample and the time-to-response, making them highly attractive for everyday clinical use.

This study aims to examine the performance of the panel Vantage in a routine inspection of non-squamous non-small lung cancer cells more (NSCLC) patients and to validate and compare the output with Solid Tumors (OST) DNA kit panel Oncomine, which is a standard technique in our institution , Two parallel multiplexing approach is based on DNA NGS and direct digital detection of DNA technology to evaluate SNVs nCounter®. A total of 42 advanced non-squamous NSCLC patients were prospectively included in this study.

ELISA kit for Human Entamoeba Histolytica
KTE63027-48T	Abbkine	48T	EUR 354
Description: Quantitative sandwich ELISA for measuring Human Entamoeba Histolytica in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Entamoeba Histolytica
KTE63027-5platesof96wells	Abbkine	5 plates of 96 wells	EUR 2252
Description: Quantitative sandwich ELISA for measuring Human Entamoeba Histolytica in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Entamoeba Histolytica
KTE63027-96T	Abbkine	96T	EUR 572
Description: Quantitative sandwich ELISA for measuring Human Entamoeba Histolytica in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Entamoeba histolytica Calreticulin
1-CSB-EP004458EKM	Cusabio	EUR 611.00 EUR 309.00 EUR 1827.00 EUR 939.00 EUR 1218.00 EUR 397.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Entamoeba histolytica Calreticulin expressed in E.coli

Entamoeba histolytica Antibody
abx021709-1ml	Abbexa	1 ml	EUR 878

Entamoeba histolytica PCR kit
PCR-H670-48D	Bioingentech	50T	EUR 453

Entamoeba histolytica PCR kit
PCR-H670-96D	Bioingentech	100T	EUR 572

Entamoeba histolytica RT PCR kit
RTq-H670-100D	Bioingentech	100T	EUR 717

Entamoeba histolytica RT PCR kit
RTq-H670-150D	Bioingentech	150T	EUR 808

Entamoeba histolytica RT PCR kit
RTq-H670-50D	Bioingentech	50T	EUR 598

Recombinant Entamoeba histolytica protein [His]
DAGF-207	Creative Diagnostics	100ug	EUR 850

Entamoeba histolytica Ag ELISA Kit
DEIA2309PY	Creative Diagnostics	96T	EUR 776
Description: The Entamoeba histolytica Ag ELISA is a device for direct detection of Entamoeba histolytica specific antigen in faecal samples.

Goat IgG (-ve control for flow cytometry) (isotype control)
20011-100	Alpha Diagnostics	100 test	EUR 103

BSA Control for AGE-BSA
35R-AA007	Fitzgerald	10 mg	EUR 224
Description: BSA Control for AGE protein (BSA modified), Cat No. 30R-AA007

BSA Control for Age-BSA
2221-BSA	Biovision		EUR 175

Core Panel Multi-Tumor control slides
TS900	Innovex	Set of 5	EUR 218

Core Panel Multi-Tumor control slides
TS900-25	Innovex	Set of 25	EUR 485

Breast Panel Multi-Tumor control slides
TS901	Innovex	Set of 5	EUR 218

Breast Panel Multi-Tumor control slides
TS901-25	Innovex	Set of 25	EUR 485

Entamoeba histolytica One-Step PCR kit
Oneq-H670-100D	Bioingentech	100T	EUR 866

Entamoeba histolytica One-Step PCR kit
Oneq-H670-150D	Bioingentech	150T	EUR 981

Entamoeba histolytica One-Step PCR kit
Oneq-H670-50D	Bioingentech	50T	EUR 718

Human Entamoeba histolytica Antigen ELISA Kit
GA-E1828HM-48T	GenAsia Biotech	48T	EUR 289

Human Entamoeba histolytica Antigen ELISA Kit
GA-E1828HM-96T	GenAsia Biotech	96T	EUR 466

Anserini Entamoeba histolytica Antigen ELISA Kit
EAE0168	Abclonal	96Tests	EUR 521

Rat Entamoeba histolytica Antigen ELISA Kit
ERE0168	Abclonal	96Tests	EUR 521

Rabbit Entamoeba histolytica Antigen ELISA Kit
ERTE0168	Abclonal	96Tests	EUR 521

Mouse Entamoeba histolytica Antigen ELISA Kit
EME0168	Abclonal	96Tests	EUR 521

Porcine Entamoeba histolytica Antigen ELISA Kit
EPE0168	Abclonal	96Tests	EUR 521

Human Entamoeba histolytica Antigen ELISA Kit
EHE0168	Abclonal	96Tests	EUR 521

Canine Entamoeba histolytica Antigen ELISA Kit
ECE0168	Abclonal	96Tests	EUR 521

Goat Entamoeba histolytica Antigen ELISA Kit
EGTE0168	Abclonal	96Tests	EUR 521

Bovine Entamoeba histolytica Antigen ELISA Kit
EBE0168	Abclonal	96Tests	EUR 521

Human Entamoeba histolytica Antigen ELISA Kit
201-12-1812	SunredBio	96 tests	EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Entamoeba Histolytica Antigen ELISA Kit
abx053908-96tests	Abbexa	96 tests	EUR 668

Canine Entamoeba histolytica Antigen ELISA KIT
QY-E70153	Qayee Biotechnology	96T	EUR 426

Human Entamoeba histolytica Antigen ELISA Kit
QY-E02359	Qayee Biotechnology	96T	EUR 361

Control for HER2, 4 cases (1.5mm)
HRC081	Pantomics	1	EUR 85
Description: IHC control array containing 4 breast cancer cases of strong, moderate, low and negative HER2 breast cancer expressers in duplicates

Control for PR, 3 cases (1.5mm)
PRC061	Pantomics	1	EUR 85
Description: IHC control array containing 3 breast cancer cases of strong, moderate, low/negative progesterone receptor (PR) expressers in duplicates

SLLK, Control Peptide for TSP1 Inhibitor
HY-P0301	MedChemExpress	1mg	EUR 133

Control for ER, 4 cases (1.5mm)
ERC081	Pantomics	1	EUR 85
Description: IHC control array containing 4 breast cancer cases of strong, moderate, weak/nil estrogen receptor (ER) expressers in duplicates

Control for DOG1, 3 cases (1.5mm)
DOG1061	Pantomics	1	EUR 85
Description: IHC control array containing 3 GIST cases of strong, moderate, weak/nil DOG1 expressers in duplicates

Control for Ckit, 6 samples (1.5mm)
CKIT061	Pantomics	1	EUR 85
Description: Gastrointestinal stroma tumors, 6 cores, 3 cases in duplicates showing strong, moderate and no expression of cKit molecule.

PAS/Glycogen, For Digestion (Control Slides)
TCS0024-100	ScyTek Laboratories	100 Slides	EUR 706

PAS/Glycogen, For Digestion (Control Slides)
TCS0024-25	ScyTek Laboratories	25 Slides	EUR 232

PAS/Glycogen, For Digestion (Control Slides)
TCS0024-5	ScyTek Laboratories	5 Slides	EUR 98

Thiostatin Rat protein, control for western
TSTN11-C	Alpha Diagnostics	100 ul	EUR 286

Control/Blocking peptide for Human WNT5a
WNT511-P	Alpha Diagnostics	100 ug	EUR 164

Entamoeba histolytica Multidrug resistance protein 1 (MDR1)
1-CSB-YP001046EKM	Cusabio	EUR 679.00 EUR 335.00 EUR 2172.00 EUR 1051.00 EUR 1442.00 EUR 435.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Entamoeba histolytica Multidrug resistance protein 1(MDR1) expressed in Yeast

Guinea Pig Entamoeba histolytica Antigen ELISA Kit
EGE0168	Abclonal	96Tests	EUR 521

Human Entamoeba Histolytica Antibody IgG ELISA Kit
abx055727-96tests	Abbexa	96 tests	EUR 668

Ferret IgG (Control, non-immune, isotype control), semi-pure for ELISA
20021-1	Alpha Diagnostics	100 ug	EUR 225

Elk IgG, semi-pure for ELISA (Control, non-immune, isotype control)
20024-1	Alpha Diagnostics	100 ug	EUR 225

Deer IgG, semi-pure for ELISA (Control, non-immune, isotype control)
20024-2	Alpha Diagnostics	100 ug	EUR 225

Bison IgG, semi-pure for ELISA (Control, non-immune, isotype control)
20025-1	Alpha Diagnostics	100 ug	EUR 225

Raccoon IgG, semi-pure for ELISA (Control, non-immune, isotype control)
20026-1	Alpha Diagnostics	100 ug	EUR 225

Skunk IgG, semi-pure for ELISA (Control, non-immune, isotype control)
20027-1	Alpha Diagnostics	100 ug	EUR 225

Holder for Plasmid Midi, Maxi and Maxi plus, Ion Exchange column
FAPDE-holder-for-ion-exchange	Favorgen	1 prep	EUR 158

Control Sections for FD NeuroSilver™ Kit
PCS101	FD Neurotechnologies	each	EUR 58

Positive Control for Anti-Rat MEH Antibody
MEHCON	Detroit R&D	100 uL	EUR 125

Positive Control for Anti-Human CYP1A2 Antibody
P1A2CON	Detroit R&D	100 uL	EUR 125

Positive Control for Anti-Rat CYP2B1 Antibody
P2B1PTCON	Detroit R&D	100 uL	EUR 125

Positive Control for Anti-Rat CYP2C Antibody
P2CCON	Detroit R&D	100 uL	EUR 125

Positive Control for Anti-Rat CYP2D Antibody
P2DCON	Detroit R&D	100 uL	EUR 125

Positive Control for Anti-Human CYP2E1 Antibody
P2E1PTCON	Detroit R&D	100 uL	EUR 125

Positive Control for Anti-Rat CYP3A2 Antibody
P3A2PTCON	Detroit R&D	100 uL	EUR 125

Positive Control for Anti-Human CYP3A Antibody
P3ACON	Detroit R&D	100 uL	EUR 125

Positive Control for Anti-Human CYP1B1 Antibody
PH1B1CON	Detroit R&D	100 uL	EUR 125

SLLK, Control Peptide for TSP1 Inhibitor(TFA)
HY-P0301A	MedChemExpress	5mg	EUR 380

Mouse IgG (-ve control for flow cytometry)
20008-1-100	Alpha Diagnostics	100 tests	EUR 103

Rabbit IgG (-ve control for flow cytometry)
20009-1-100	Alpha Diagnostics	100 tests	EUR 103

Purified Flagellin (salmonella) protein control for WB
FLGN17-C	Alpha Diagnostics	100 ul	EUR 286

Purified, Bovine S100 protein control for WB
S1001-C	Alpha Diagnostics	100 ul	EUR 286

Purified, Human S100 protein control for WB
S1002-C	Alpha Diagnostics	100 ul	EUR 286

Control/Blocking peptide for Mouse Vimentin (Vim)
VIM11-C	Alpha Diagnostics	100 ug	EUR 164

Control/Blocking peptide for Human WNT-1
WNT111-P	Alpha Diagnostics	100 ug	EUR 457

Chicken Egg Ovalbumin protein control for Western
OVA11-C	Alpha Diagnostics	100 ul	EUR 286

Mouse negative control sera (purified) for IHC
NCM802M	Innovex	7 ml	EUR 199

Control/Blocking peptide for Ephrin-B2 antibody
NIVE2B-C	Alpha Diagnostics	100 ug	EUR 164

Purified bovine Lactoferrin protein control for Western
LTF13-C	Alpha Diagnostics	100 ul	EUR 286

Haptoglobin Human Plasma protein control for WB
HGLB18-C	Alpha Diagnostics	100 ul	EUR 286

Human Hemopexin purified protein control for Western
HPEX11-C	Alpha Diagnostics	100 ul	EUR 286

Hemopexin Mouse, purified protein control for Western
HPEX16-C	Alpha Diagnostics	100 ul	EUR 286

Rec. Human CD21 protein control for WB
CD21-C	Alpha Diagnostics	100 ul	EUR 286

Mouse Ceruloplasmin protein control for western blot
CP16-C	Alpha Diagnostics	100 ul	EUR 286

Rat Ceruloplasmin protein control for western blot
CP17-C	Alpha Diagnostics	100 ul	EUR 286

Bovine Adenosine deaminase protein control for Western
ADA11-C	Alpha Diagnostics	100 ul	EUR 286

Human Apolipoprotein B protein control for WB
APOB21-C	Alpha Diagnostics	100 ul	EUR 286

Human recombinant purified matrilysin/MMP7 (matrix metalloproteinase 7) protein control for WB
MMP71-C	Alpha Diagnostics	100 ul	EUR 286

Control siRNA Vector (pGB-control)
9500C-20	Biovision		EUR 338

Control sections for FD NeuroApop™ Kit (frozen)
PCS102-	FD Neurotechnologies	each	EUR 73

Control sections for FD NeuroApop™ Kit (paraffin)
PCS102-1	FD Neurotechnologies	each	EUR 67.03
Description: Best quality products

Control sections for FD NeuroApop™ Kit (frozen)
PCS102-2	FD Neurotechnologies	each	EUR 67.12
Description: Best quality products

Positive Control for Anti-Rat CYP1A1/1A2 Antibody
P1ACON	Detroit R&D	100 uL	EUR 125

Positive Control for Anti-Rat CHP2B1/2 Antibody
P2B12PTCON	Detroit R&D	100 uL	EUR 125

Mouse Anti-Cardiolipin Ig's -ve control for ELISA
5502	Alpha Diagnostics	1 ml	EUR 225

Positive Control for Anti-Human Cytochrome b5 Antibody
Cb5CON	Detroit R&D	100 uL	EUR 125

Purified Human Neutrophil Elastase protein control for WB
ESTE11-C	Alpha Diagnostics	100 ul	EUR 286

Human Salivary Amylase protein positive control for WB
SAMY16-C	Alpha Diagnostics	100 ul	EUR 286

Purified Human Survivin protein for WB +ve control
SURV11-C	Alpha Diagnostics	100 ul	EUR 286

Purified, recombinant Human TACE control protein for WB
TACE11-C	Alpha Diagnostics	100 ul	EUR 286

Human Pancreatic Amylase protein control for Western blot
PAMY13-C	Alpha Diagnostics	100 ul	EUR 286

Human Prothrombin (Factor II) protein control for Western
PRTN11-C	Alpha Diagnostics	100 ul	EUR 286

Mouse Prothrombin (Factor II) protein control for Western
PRTN13-C	Alpha Diagnostics	100 ul	EUR 286

Myoglobin, Human Cardiac, purified protein control for western
MYG17-C	Alpha Diagnostics	100 ul	EUR 286

Purified Nipah virus Glycoprotein control for Western Blotting
NIV11-C	Alpha Diagnostics	100 ul	EUR 286

Purified Nipah virus Nucleoprotein control for Western Blotting
NIV21-C	Alpha Diagnostics	100 ul	EUR 225

Purified Lassa fever Nucleoprotein control for Western Blotting
LFNP15-C-10	Alpha Diagnostics	100 ul	EUR 286

Control/Blocking peptide for Mouse Histone deacetylase 1
HDAC111-P	Alpha Diagnostics	100 ug	EUR 164

Purified human Catalase (CATL) protein control for WB
CATL11-C	Alpha Diagnostics	100 ul	EUR 286

Chicken Egg Conalbumin (ovotransferrin) protein control for Western
CECA15-C	Alpha Diagnostics	100 ul	EUR 286

Chicken Egg white proteins total control for Western
CEGW12-C	Alpha Diagnostics	100 ul	EUR 286

Chicken Egg yellow proteins total control for Western
CEYP12-C	Alpha Diagnostics	100 ul	EUR 286

Chicken Ovomucoid (trypsin inhibitor) protein control for Western
COVM13-C	Alpha Diagnostics	100 ul	EUR 286

Purified Cholera Toxin protein control for Western Blot
CTOX11-C	Alpha Diagnostics	100 ul	EUR 286

Rabbit Beta Actin (muscle) protein control for Western
ACTB15-C	Alpha Diagnostics	100 ul	EUR 324

Chicken Serum Albumin protein control for Western blot
ALBC15-C	Alpha Diagnostics	100 ul	EUR 286

Monkey (Rhesus) Serum Albumin protein control for WB
ALBMK21-C	Alpha Diagnostics	100 ul	EUR 286

Monkey (Rhesus) Serum Albumin protein control for WB
ALBMK22-C	Alpha Diagnostics	100 ul	EUR 286

Human Apolipoprotein C-I protein control for WB
APOC11-C	Alpha Diagnostics	100 ul	EUR 286

Human Apolipoprotein C-II protein control for WB
APOC22-C	Alpha Diagnostics	100 ul	EUR 286

Human Apolipoprotein C-III protein control for WB
APOC32-C	Alpha Diagnostics	100 ul	EUR 286

Human plasma Apolipoprotein E protein control for WB
APOE13-C	Alpha Diagnostics	100 ul	EUR 286

Human Plasma Apolipoprotein J protein control for WB
APOJ13-C	Alpha Diagnostics	100 ul	EUR 286

Recombinant Beta Lactamase protein control for Western blot
BLAC11-C	Alpha Diagnostics	100 ul	EUR 286

Quality Control
abx098966-1vial	Abbexa	1 vial	EUR 300

pMD18- Control
PVT10563	Lifescience Market	2 ug	EUR 266

pMD19- Control
PVT10564	Lifescience Market	2 ug	EUR 266

NATtrol Rotavirus (Stool Matrix) (0.5 mL)
NATROTA-GP	Zeptometrix	0.5 mL	EUR 93.68
Description: Please contact Gentaur in order to receive the datasheet of the product.

Positive Control for anti-GST p-forms: rat P
GSTCON3	Detroit R&D	100 uL	EUR 125

Mouse Monoclonal Anti-Cardiolipin Ig's +ve control for ELISA
5503	Alpha Diagnostics	1 ml	EUR 286

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNC041520-100	Biotium	100uL	EUR 199
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),CF405S conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNC041520-500	Biotium	500uL	EUR 544
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),CF405S conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNC471520-100	Biotium	100uL	EUR 199
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),CF647 conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNC471520-500	Biotium	500uL	EUR 544
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),CF647 conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNCAP1520-100	Biotium	100uL	EUR 199
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNCAP1520-500	Biotium	500uL	EUR 544
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),Alkaline Phosphatase conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNC801520-100	Biotium	100uL	EUR 199
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),CF680 conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNC801520-500	Biotium	500uL	EUR 544
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),CF680 conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNC811520-100	Biotium	100uL	EUR 199
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),CF680R conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNC811520-500	Biotium	500uL	EUR 544
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),CF680R conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNC681520-100	Biotium	100uL	EUR 199
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),CF568 conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNC681520-500	Biotium	500uL	EUR 544
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),CF568 conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNC701520-100	Biotium	100uL	EUR 199
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),CF770 conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNC701520-500	Biotium	500uL	EUR 544
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),CF770 conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNC881520-100	Biotium	100uL	EUR 199
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),CF488A conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNC881520-500	Biotium	500uL	EUR 544
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),CF488A conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNC611520-100	Biotium	100uL	EUR 199
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),CF660R conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNC611520-500	Biotium	500uL	EUR 544
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),CF660R conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNC941520-100	Biotium	100uL	EUR 199
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),CF594 conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNC941520-500	Biotium	500uL	EUR 544
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),CF594 conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNCA1520-250	Biotium	250uL	EUR 383
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),APC conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNUB1520-100	Biotium	100uL	EUR 209
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R), Concentration: 0.2mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNUB1520-500	Biotium	500uL	EUR 458
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R), Concentration: 0.2mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNUM1520-50	Biotium	50uL	EUR 395
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R), 1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNCH1520-100	Biotium	100uL	EUR 199
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNCH1520-500	Biotium	500uL	EUR 544
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),Horseradish Peroxidase conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNC051520-100	Biotium	100uL	EUR 199
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),CF405M conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNC051520-500	Biotium	500uL	EUR 544
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),CF405M conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNCP1520-250	Biotium	250uL	EUR 383
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),PerCP conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNCR1520-250	Biotium	250uL	EUR 383
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),RPE conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNC401520-100	Biotium	100uL	EUR 199
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),CF640R conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNC401520-500	Biotium	500uL	EUR 544
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),CF640R conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNC431520-100	Biotium	100uL	EUR 199
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),CF543 conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNC431520-500	Biotium	500uL	EUR 544
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),CF543 conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNCB1520-100	Biotium	100uL	EUR 199
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),Biotin conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNCB1520-500	Biotium	500uL	EUR 544
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),Biotin conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNC551520-100	Biotium	100uL	EUR 199
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),CF555 conjugate, Concentration: 0.1mg/mL

Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R) Antibody
BNC551520-500	Biotium	500uL	EUR 544
Description: Primary antibody against Negative Control for Rabbit Monoclonal Antibodies (NCRBM/1520R),CF555 conjugate, Concentration: 0.1mg/mL

Control/Blocking peptide for Mouse TATA box-binding protein
TATAB11-C	Alpha Diagnostics	100 ug	EUR 164

Control/Blocking peptide for Tobacco Mosaic Virus Movement Protein
TMVMP11-P	Alpha Diagnostics	100 ug	EUR 164

Troponin-I, Recombinant, Human Cardiac, protein control for WB
TNIC15-C	Alpha Diagnostics	100 ul	EUR 286

Troponin-T, Human Cardiac, pure protein control for WB
TNTC55-C	Alpha Diagnostics	100 ul	EUR 286

Zika Virus prM Protein (African) control for Western blot
ZPRM11-C	Alpha Diagnostics	100 ul	EUR 286

Human Native Retinol binding protein (RBP) control for Western
RBP41-C	Alpha Diagnostics	100 ul	EUR 286

Prealbumin (Transeryhtin/TTR) Human, protein control for Western blot
PALB11-C	Alpha Diagnostics	100 ul	EUR 286

Recombinant purified human Parvalbumin (PARV) protein control for Western
PARV11-C	Alpha Diagnostics	100 ul	EUR 286

Recombinant, purified, Rat Parvalbumin (PARV) protein control for Western
PARV12-C	Alpha Diagnostics	100 ul	EUR 286

Recombinant Mycoplasma Pulmonis (Mp) protein control for Western Blot
MPUL11-C	Alpha Diagnostics	100 ul	EUR 445

Overall, 95% of the sample was marked with both technologies. Vantage panels accounted for 95% sensitivity and 82% specificity. In terms of predictive values, the probability of actually variants SNV when detected by Ncounter panel was 82%, whereas the probability of not variants SNV if not detected by the platform is 95%. Finally, Cohen’s Kappa coefficient was 0.76, indicating a substantial class correlation between OST and the Vantage panel. Our results make Ncounter a practical and cost-effective means of analytical sensitivity.